Simultaneous detection of enterovirus 71 and coxsackievirus A16 using dual-colour upconversion luminescent nanoparticles as labels.
نویسندگان
چکیده
We highlight a novel fluorescence analysis for sensitive and selective detection of EV-71 and CV-A16 by combining labelling technology based on dual-colour upconversion fluorescence nanoparticles (UCNPs) with magnetic bioseparation and concentration technology based on magnetite nanoparticles (MNPs).
منابع مشابه
Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: Improved primers for the specific detection of human enterovirus 71 by RT PCR
BACKGROUND Human enterovirus 71 has emerged as an important pathogen in the Asia Pacific region and it is important to be able to make a rapid and specific diagnosis for outbreak control. Recent Asian strains of Coxsackievirus A16 have changes in the VP1 gene which causes mispriming of widely used primers for human enterovirus 71 specific identification. METHODS Local strains of Coxsackieviru...
متن کاملDevelopment of a multiplex polymerase chain reaction assay for simultaneous identification of human enterovirus 71 and coxsackievirus A16
Human enterovirus 71 (HEV71) and coxsackievirus A16 (CVA16) are two major aetiological agents of hand, foot and mouth disease (HFMD) in children. Recently there have been several large outbreaks of HFMD in Vietnam and the Asia-Pacific region. In this study, a multiplex RT-PCR assay was developed in order to detect simultaneously HEV71, CVA16 and other human enteroviruses. Enterovirus detection ...
متن کاملNovel substituted heteroaromatic piperazine and piperidine derivatives as inhibitors of human enterovirus 71 and coxsackievirus a16.
A series of substituted heteroaromatic piperazine and piperidine derivatives were found through virtual screening based on the structure of human enterovirus 71 capsid protein VP1. The preliminary biological evaluation revealed that compounds 8e and 9e have potent activity against EV71 and Coxsackievirus A16 with low cytotoxicity.
متن کاملExternal quality assessment for enterovirus 71 and coxsackievirus A16 detection by reverse transcription-PCR using armored RNA as a virus surrogate.
Three armored RNAs (virus-like particles [VLPs]) containing target sequences from enterovirus 71 (EV71) and coxsackievirus A16 (CA16) and a pan-enterovirus (pan-EV) sequence were constructed and used in an external quality assessment (EQA) to determine the performance of laboratories in the detection of EV71 and CA16. The EQA panel, which consisted of 20 samples, including 14 positive samples w...
متن کاملMolecular Detection of Human Enterovirus 71 Causing Hand, Foot and Mouth Disease in Klang Valley, Malaysia
Hand, Foot and Mouth Disease (HFMD) is a childhood infection caused by Enterovirus 71 (EV71) or Coxsackievirus A16 (CA16). Occasionally, Enterovirus 71 becomes fatal and causes encephalitis. The objective of the present study was to determine HFMD caused by Enterovirus 71 if prevalent in the Klang Valley, Malaysia. 184 specimens were collected from the patients reported to University Kebangsaan...
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ورودعنوان ژورنال:
- Chemical communications
دوره 48 40 شماره
صفحات -
تاریخ انتشار 2012